You can download my research publications below.
Microfluidic LC related
2D LC related
Comparison of orthogonality estimation methods for two-dimensiona separation of peptides, M. Gilar, J. Fridrich, M.R. Schure, A. Jaworski, Analytical Chemistry, 2012, 84, 8722-8732. 2012 AC 2DLC orthogonality.pdf (724,5 kB). Supplemental data are here: Supplemental data S2 S4 S5.xlsx (158,3 kB) and Supplemental S3.docx (60,3 kB). The supplemental orthogonality calculator S1 is too large for this site. You can download it from here.
Retention behavior of peptides in hydrophilic-interaction chromatography, M. Gilar, A. Jaworski, Journal of Chromatography A, 2011, 1218, 8890-8896. 2011 JCA HILIC pep ret.pdf (720 kB)
Comparison of 1-D and 2-D LC MS/MS methods for proteomic analysis of human serum, M. Gilar, P. Olivova, A.B. Chacraborty, A. Jaworski, S.J. Geromanos, J.C. Gebler, Electrophoresis, 2009, 30, 1157-1167. 2009 ELPHO 2D LC serum.pdf (1235211)
Mixed-mode chromatography for fractionation of peptides, phosphopeptides and sialylated glycopeptides, M.Gilar, Y.Q. Yu, J. Ahn, J. Fournier, J.C. Gebler, Journal of Chromatography A, 2008, 1191, 162-170. 2008 JCA MMC glyco phospho.pdf (561,4 kB)
Peptide retention prediction applied to proteomic data analysis, M. Gilar, A. Jaworski, P. Olivova, J.C. Gebler, Rapid communications in Mass Spectrometry, 2007, 21, 2813-2821. 2007 RCM pep ret. pred.pdf (2,9 MB)
Orthogonality of separation in two-dimensional liquid chromatography, M. Gilar, P. Olivova, A.E. Daly, J.C. Gebler, Analytical Chemistry, 2005, 77, 6426-3464. 2005 ACH 2DLC.pdf (295,3 kB)
Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimension, M. Gilar, P. Olivova, A.E. Daly, J.C. Gebler, Journal of Separation Science, 2005, 28, 1694-1703. 2005 JSS 2DLC.pdf (442,3 kB)
Implications of column peak capacity on the separation of complex peptide mixtures in single- and two-dimensional high-performance liquid chromatography, M. Gilar, A.E. Daly, M. Kele, U.D. Neue, J.C. Gebler, Journal of Chromatography A, 2004, 1061, 183-192. 2004 JCA peak capacity.pdf (588,3 kB)
Glycan and glycopeptide analysis
Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectrometry, M. Gilar, Y.Q. Yu, J. Ahn, H. Xie, H. Han, W. Ying, X. Qian, Analytical Biochemistry, 2011, 417, 80-88. 2011 ABIO Gilar glycopep personal copy.pdf (1,3 MB)
Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7 um sorbent, J. Ahn, J. Bones, Y.Q. Yu, P.M. Rudd, M. Gilar, Journal of Chromatography B, 2010, 878, 403-408. 2010 JCHB Ahn HILIC glycans.pdf (804,6 kB)
Identification of N-linked glycosylation sites using glycoprotein digestion with Pronase prior to MALDI tandem time-of-flight mass spectrometry, Y.Q. Yu, J. Fournier, M. Gilar, J.C. Gebler, Analytical Chemistry, 2007, 79, 1731-1738. 2007 ACH pronase glycopep.pdf (204,5 kB)
A rapid sample preparation method for mass spectrometric characterization of N-linked glycans, Y.Q. Yu, M. Gilar, J. Kaska, J.C. Gebler, Rapid Communications in Mass Spectrometry, 2005, 19, 2331-2336. 2005 RCM Glycans Yu.pdf (863,4 kB)
HPLC, CE, peptides, proteins, oligonucleotides
Solvent selectivity and strength in reversed-phase liquid chromatography separation of peptides, M. Gilar, A. Jaworski, T.S. McDonalds, Journal of Chromatography A, 2014, 1337, 140-146. JCA 2013 solv strength select pep.pdf (333,1 kB). Supplemental material: Gilar Supplement S1.xls (3.6 MB); Gilar Supplement S5.xls (38,4 kB); Gilar Supplement S4.xls (372,2 kB); Gilar Supplement S3.xls (135,7 kB); Gilar Supplement S2.xls (105,0 kB)
Rapid comparison of a candidate biosimilar to an innovator monoclonal antibody with advanced liquid chromatography and mass spectrometry technologies, H. Xie, A. Chacraborty, J. Ahn, Y.Q. Yu, D.P. Dakshinamoorthy, M. Gilar, W. Chen, St.J. Skilton, J.R. Mazzeo, mAbs, 2010, 2, 379-394. 2010 MABS Xie p.pdf (854,7 kB)
Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and UPLC/MS(E) analysis of RNA oligonucleotides. V.B. Ivleva, Y.Q. Yu, M. Gilar, Rapid Communications in Mass Spectrometry, 2010, 24, 2631-2640. 2010 RCM oligo MSMS.pdf (828,5 kB)
Utility of retention prediction model for investigation of peptide separation selectivity in reversed-phase liquid chromatography: Impact of concentration of trifluoroacetic acid, column temperature, gradient slope and type of stationary phase, M. Gilar, H. Xie, A. Jaworski, Analytical Chemistry, 2010, 82, 265-275. 2010 ACH pep RP selectivity.pdf (1,6 MB)
Reversed-phase ion-pair liquid chromatography analysis and purification of small interfering RNA, S.M. McCarthy, M. Gilar, J.C. Gebler, Analytical Biochemistry, 2009, 390, 181-188. 2009 ABIO siRNA.pdf (1,6 MB)
Characterization of protein impurities and site-specific modifications using peptide mapping with liquid chromatography and data independent acquisition mass spectrometry, H. Xie, M. Gilar, J.C. Gebler, Analytical Chemistry, 2009, 81, 5699-5708. 2009 ACH xie pep map.pdf (947,8 kB)
Phosphopeptide enrichment using microscale titanium dioxide solid phase extraction, Y.Q. Yu, J. Fournier, M. Gilar, J.C. Gebler, Journal of Separation Science, 2009, 32, 1189-1199. 2009 JSS phosphopepTiO2.pdf (905,8 kB)
Peak capacity in gradient reversed-phase liquid chromatography of biopolymers: Theoretical and practical implications for the separation of oligonucleotides, M. Gilar, U.D. Neue, Journal of Chromatography A, 2007, 1169, 139-150. 2007 JCHA UPLC peak capac.pdf (371,6 kB)
A complete peptide mapping of membrane proteins: a novel surfactant aiding the enzymatic digestion of bacteriorhodopsin, Y.Q. Yu, M. Gilar, J.C. Gebler, Rapid Communications in Mass Spectrometry, 2004, 18, 711-715. 2004 RCM membrane Rapigest.pdf (132,2 kB)
Electrospray ionization mass spectrometric analysis of nucleic acids using high-throughput on-line desalting, K.J. Fountain, M. Gilar, J.C. Gebler, Rapid Communications in Mass Spectrometry, 2004, 18, 1295-1302. 2004 RCM fast LC-MS oligo.pdf (232,9 kB)
Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins, Y.Q. Yu, M. Gilar, P.J. Lee, E.S.P. Bouvier, J.C. Gebler, Analytical Chemistry, 2003, 75, 6023-6028. 2003 ACH rapigest.pdf (106,8 kB)
Characterization of therapeutic oligonucleotides using liquid chromatography with on-line mass spectrometry detection, M. Gilar, K.J. Fountain, Y. Budman, J.L. Holyoke, H. Davoudi, J.C. Gebler, Oligonucleotides, 2003, 13, 229-243. 2003 oligon LCMS antis.pdf (255,3 kB)
Analysis of native and chemically modified oligonucleotides by tandem ion-pair reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry. K.J. Fountain, M. Gilar, J.C. Gebler, Rapid Communications in Mass Spectrometry, 2003, 17, 646-653. 2003 RCM LCMS oligo.pdf (160,4 kB)
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography, K.J. Fountain, M. Gilar, Y. Budman, J.C. Gebler, Journal of Chromatography B, 783, 61-72. 2003 JCHB fluor oligo.pdf (598 kB)
Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: Retention prediction, M. Gilar, K.J. Fountain, Y. Budman, U.D. Neue, K.R. Yardley, P.D. Raniville, R.J. Russel, J.C. Gebler, Journal of Chromatography A, 2002, 958, 167-182. 2002 JCA oligo ret pred.pdf (696,4 kB)
High-throughput biopolymer desalting by solid-phase extraction prior to mass spectrometric analysis, M. Gilar, A. Belenky, B.H. Wang, 2001, 921, 3-13. 2001 JCHA HT desalt.pdf (261,7 kB)
Analysis and purification of synthetic oligonucleotides by rversed-phase high-performance liquid chromatography with photodiode array and mass spectrometry detection, Analytical Biochemistry, M. Gilar, 2001, 298, 196-206. 2001 ABIO.pdf (113,4 kB)
Polymer solutions as pseudostationary phase for capillary electrochromatographic separation of DNA diastereomers. M. Gilar, A. Belenky, A.S. Cohen, Electrophoresis, 2000, 21, 2999-3009. 2000 ELPHO diastereo.pdf (142,9 kB)
Purification of crude DNA oligonucleotides by solid phase extraction and reversed-phase high-performance liquid chromatography, Journal of Chromatography A, M. Gilar, E.S.P. Bouvier, 2000, 890, 167-177. 2000 JCHA DMT ON SPE.pdf (555,6 kB)
Impact of 3'-exonuclease stereoselectivity on the kinetics of phosphorothioate oligonucleotide metabolism, M. Gilar, A. Beleky, Y. Budman, D.L. Smisek, A.S. Cohen, Antisense and nucleic acid drug development, 1998, 8, 35-42. Antis1998Gilar.PDF (787,5 kB)
Study of phosphorothioate-modified oligonucleotide resistance to 3'-exonuclease using capillary electrophoresis, M. Gilar, A. Beleky, Y. Budman, D.L. Smisek, A.S. Cohen, Journal of Chromatography B, 1998, 714, 13-20. Gilar_jchB1998_PS_CE.PDF (741,8 kB)
Kinetics of phosphorothioate oligonucleotide metabolism in biological fluids, M. Gilar, A. Beleky, D.L. Smisek, A. Bourque, A.S. Cohen, Nucleic Acids Research, 1997, 25, 3615-3620. NAR_Gilar_1997.pdf (101,3 kB), missing Fig. 3B NAR_Gilar_1997 fig3B.pdf (10,9 kB)
Chiral separations
Enantiomer separation of dihydropyridine calcium antagonists with cyclodextrines as chiral selectors: structural correlation, M. Gilar, M. Uhrova, E. Tesarova, Journal of Chromatography B, 1996, 681, 133-141. JCHB 1996 CE model.pdf (666,5 kB)
Correlation between structure of dihydropyridine calcium antagonists and their retention behavior and enantioseparation on the beta-cyclodextrine stationary phase in HPLC, Journal of High Resolution Chromatography, E. Tesarova, M. Gilar, P. Hobza, M. Kabelac, Z. Deyl, E. Smolkova-Keulemansova, 1995, 18, 597-601. HRC 1995 CD modeling.PDF (372,6 kB)
Study of retention behaviour and enantioseparation of selected calcium antagonists on cyclodextrine stationary phases in high performance liquid chromatography, M. Gilar, E. Tesarova, Z. Deyl, STP Pharma Sciences, 1995, 5, 409-414. STP pharm sci 1995.pdf (534,2 kB)
